Congenic - Wikipedia

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Generating congenic strains Congenic FromWikipedia,thefreeencyclopedia Jumptonavigation Jumptosearch Taxonomicdescription Nottobeconfusedwithcongeners–seeconspecificity. Ingenetics,twoorganismsthatdifferinonlyonelocusandalinkedsegmentofchromosomearedefinedascongenic.[1]Similarly,organismsthatarecoisogenicdifferinonelocusonlyandnotinthesurroundingchromosome.Unlikecongenicorganisms,coisogenicorganismscannotbebredandonlyoccurthroughspontaneousortargetedmutationatthelocus. Contents 1Generatingcongenicstrains 2Seealso 3Notesandreferences 4Furtherreading Generatingcongenicstrains[edit] Congenicstrainsaregeneratedinthelaboratorybymatingtwoinbredstrains(usuallyratsormice),andback-crossingthedescendants5–10generationswithoneoftheoriginalstrains,knownastherecipientstrain.Typicallyselectionforeitherphenotypeorgenotypeisperformedpriortoeachback-crossgeneration.Inthismannereitheraninterestingphenotype,oradefinedchromosomalregionassayedbygenotype,ispassedfromthedonorstrainontoanotherwiseuniformrecipientbackground.Congenicmiceorratscanthenbecomparedtothepurerecipientstraintodeterminewhethertheyarephenotypicallydifferentifselectionwasforagenotypicregion,ortoidentifythecriticalgeneticlocus,ifselectionwasforaphenotype. Speedcongenicscanbeproducedinaslittleasfiveback-crossgenerations,[2]throughtheselectionateachgenerationofoffspringthatnotonlyretainthedesiredchromosomalfragment,butalso'lose'themaximumamountofbackgroundgeneticinformationfromthedonorstrain.Thisisalsoknownasmarker-assistedcongenics,duetotheuseofgeneticmarkers,typicallymicrosatellitemarkers,butnow,morecommonly,singlenucleotidepolymorphismmarkers(SNPs).Theprocesscanbefurtheraidedbythesuperovulationoffemales,toproducemanymoreeggs. Seealso[edit] Geneknockout Notesandreferences[edit] ^"Congenic-definitionfromBiology-Online.org".Biology-Online.orgdictionary.Biology-Online.org.Retrieved25August2017. ^MarkelP,ShuP,EbelingC,et al.(November1997)."Theoreticalandempiricalissuesformarker-assistedbreedingofcongenicmousestrains".Nat.Genet.17(3):280–284.doi:10.1038/ng1197-280.PMID 9354790.S2CID 25212164. Furtherreading[edit] CongenicstrainsarediscussedindetailinLeeSilver'sonlinebookMouseGenetics:ConceptsandApplications: "MouseGenetics:ConceptsandApplications".LeeSilver.Retrieved2007-10-18.[1] Thisgeneticsarticleisastub.YoucanhelpWikipediabyexpandingit.vte Retrievedfrom"https://en.wikipedia.org/w/index.php?title=Congenic&oldid=1065184110" Categories:GeneticsGeneticsstubsHiddencategories:ArticleswithshortdescriptionShortdescriptionmatchesWikidataAllstubarticles Navigationmenu Personaltools NotloggedinTalkContributionsCreateaccountLogin Namespaces ArticleTalk English Views ReadEditViewhistory More Search Navigation MainpageContentsCurrenteventsRandomarticleAboutWikipediaContactusDonate Contribute HelpLearntoeditCommunityportalRecentchangesUploadfile Tools WhatlinkshereRelatedchangesUploadfileSpecialpagesPermanentlinkPageinformationCitethispageWikidataitem Print/export DownloadasPDFPrintableversion Languages DeutschفارسیItalianoNederlands日本語 Editlinks

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