DNase I, RNase-free (1 U/µL) - Thermo Fisher Scientific

Thermo Scientific DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- ... PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search HomeShopAllProductsEnzymesandInhibitorsModifyingEnzymesDNaseI,RNase-free(1U/µL)ThermoScientific™DNaseI,RNase-free(1U/µL)Catalognumber:EN0521Relatedapplications:RealTimePCR(qPCR)ReverseTranscriptionInVitroTranscriptionTechnicalSupportCustomerServiceThermoScientific™DNaseI,RNase-free(1U/µL)Catalognumber:EN0521Relatedapplications:RealTimePCR(qPCR)ReverseTranscriptionInVitroTranscriptionTechnicalSupportCustomerServiceCatalogNumberEN0521UnitSize1,000unitsPrice(SGD)RequestAQuoteCatalogNumberUnitSizePrice(SGD)EN05211,000unitsRequestAQuoteProductOverviewFiguresVideosDocumentsFAQCitations&ReferencesAdditionalInformationRecommendationsRecommendationsThermoScientificDNaseI,RNase-freeisanendonucleasethatdigestssingle-anddouble-strandedDNA.Ithydrolyzesphosphodiesterbondsproducingmono-andoligodeoxyribonucleotideswith5'-phosphateand3'-OHgroups.TheenzymeactivityisstrictlydependentonCa2+andisactivatedbyMg2+orMn2+ions.InthepresenceofMg2+,DNaseIcleaveseachstrandofdsDNAindependentlyinastatisticallyrandomfashion.InthepresenceofMn2+,theenzymecleavesbothDNAstrandsatapproximatelythesamesite,producingDNAfragmentswithblunt-endsorwithoverhangterminiofonlyoneortwonucleotides.Highlights•Recombinantenzyme•Purifiedfromnon-animalhostwithalowerlevelofintrinsicRNasesApplications•PreparationofDNA-freeRNA•RemovaloftemplateDNAfollowinginvitrotranscription•PreparationofDNA-freeRNApriortoRT-PCRandRT-qPCR•DNAlabelingbynick-translationinconjunctionwithDNAPolymeraseI•StudiesofDNA-proteininteractionsbyDNaseI,RNase-freefootprinting•GenerationofalibraryofrandomlyoverlappingDNAinserts.ReactionbuffercontainingMn2+isusedNoteDNaseIissensitivetophysicaldenaturation.Mixgentlybyinvertingthetube.Donotvortex.ForResearchUseOnly.Notforuseindiagnosticprocedures.SpecificationsConcentration1U/μLEnzymeDNaseCompatibleBufferReactionBufferQuantity1000UProductTypeDNaseIRNaseFreeFiguresDNaseIactivityinthepresenceofMg2+orMn2+ Signin Don'thaveanaccount? CreateAccount CustomProducts&Projects InstrumentManagement Account CheckOrderStatus PopularPopular TaqManReal-TimePCRAssays Antibodies Oligos,Primers&Probes GeneArtGeneSynthesis CellCulturePlasticsApplications&TechniquesSeeLinksForApplications&TechniquesBrandsSeeLinksForBrandsThermoScientificAppliedBiosystemsInvitrogenFisherScientificUnityLabServicesPatheonPPDLifeSciencesSeeLinksForLifeSciencesAntibodiesCellAnalysisCellCulture&TransfectionCloningDNA&RNAExtraction&AnalysisEpigenetics&ncRNAResearchFlowCytometryGeneExpressionAnalysis&GenotypingGenomeEditingMicroarrayAnalysisOligos,Primers,Probes&GenesPCRProteinBiologyReal-TimePCRRNAiSequencingStemCellResearchSyntheticBiologyIndustrial&AppliedScienceSeeLinksForIndustrial&AppliedScienceAgrigenomicsAnimalHealthBioprocessingBiotechCompanySolutionsCement,Coal&MineralsChromatographyDrugDiscovery&DevelopmentElectronMicroscopyEnvironmentalFood&BeverageForensicsGeosciencesManufacturing&ProcessingMassSpectrometryMaterialsScienceMicrobiologyPharma&BiopharmaRadiationDetection&MeasurementSafetyandSecurityThreatDetectionSpectroscopy,Elemental&IsotopeAnalysisSemiconductorAnalysisClinical&DiagnosticsSeeLinksForClinical&DiagnosticsAllergyDiagnosticsAnatomicalPathologyAutoimmuneDiagnosticsBiobankingCancerResearchCell&GeneTherapyClinicalGenomicsClinicalMassSpectrometryClinicalMicrobiologyClinical&TranslationalResearchDiagnosticDevelopmentDiagnosticTestingPrecisionMedicinePreclinicaltoCompanionDiagnosticDevelopmentPublicHealthLabSolutionsSeeLinksForLabSolutionsChemicalsLabEquipmentLabPlasticware&SuppliesSampleStorage&ManagementDigitalSolutionsSeeLinksForDigitalSolutionsLabInformaticsConnectPlatformInstrumentConnectivityApps&AnalysisSoftwareLabAutomationDigitalServicesDigitalCommerceShopAllProductsSeeLinksForShopAllProducts     MostPopularProductsBeakersLabCentrifugesUltraLow–TemperatureFreezersLipofectamineReagentsNalgeneLabwareOligos,Primers,Probes&GenesPipettes&PipetteTipsSuperScriptReverseTranscriptaseTaqManReal-TimePCRAssaysTRIzolReagentsAntibodiesELISAKitsMostPopularCategoriesMicroplatesPCREquipmentandSuppliesMolecularBiologyReagentsandKitsChromatographyColumnsandCartridgesChromatographySuppliesCellCultureMediaMicrobiologicalMediaandMediaAdditivesAntibioticsandSupplementsCellAnalysisProductsGelElectrophoresisEquipmentandSuppliesWesternBlotProductsMicroplateReadersandAccessoriesSpectroscopyOrderToolsHowtoOrderQuickOrderTrackYourOrderOrderHistoryOrderSupportContactUsShopAllProductsSeeLinksForShopAllProductsProductSelectionGuidesNewProductsPromotionsOnlineOffersServicesSeeLinksForServicesCustomServicesEnterprise-LevelLabInformaticsInstrumentServicePlansFinancial&LeasingSolutionsInstrumentServicesOEM&CommercialSupplyTrainingServicesCDMO&ClinicalTrialServicesSupportSeeLinksForSupportProductDocumentationManualsSafetyDataSheets(SDS)CertificatesofAnalysis&ConformanceCEDeclarationsofConformityRoHSCertificatesManufacturingSiteISOCertificationsOtherProductInformationCitations&ReferencesProductLiteratureVectorMapsSpectralDataMediaFormulationsProductSupportKnowledgeBase&FAQsInstrumentSupportSupportCentersContactUsEducationalResourcesLearningCentersConnecttoScienceDigitalTransformationGibcoCellCultureBasicsProteinMethodsLibrarySupplementalProtocolsNewsletters&JournalsTrainingServicesEventsPopularTools&CalculatorsLabAppsforMobileDevicesOligosTools&UtilitiesHubTmCalculatorforPCRMolecularBiologyToolsGibcoMediaFormulationToolCellCultureUsefulNumbersFluorescenceSpectraViewerFlowCytometryPanelBuilderCellStainingToolProteinGelConversionToolSwitchtoNuncPlasticsToolTransfectionProtocolCalculator