Deoxyribonuclease I - Wikipedia

DNase I is a nuclease that cleaves DNA preferentially at phosphodiester linkages adjacent to a pyrimidine nucleotide, yielding 5'-phosphate-terminated ... DeoxyribonucleaseI FromWikipedia,thefreeencyclopedia Jumptonavigation Jumptosearch DNASE1AvailablestructuresPDBOrthologsearch:PDBeRCSBListofPDBidcodes4AWNIdentifiersAliasesDNASE1,DNL1,DRNI,deoxyribonucleaseI,deoxyribonuclease1ExternalIDsOMIM:125505MGI:103157HomoloGene:3826GeneCards:DNASE1Genelocation(Human)Chr.Chromosome16(human)[1]Band16p13.3Start3,611,728bp[1]End3,680,143bp[1]Genelocation(Mouse)Chr.Chromosome16(mouse)[2]Band16A1|162.37cMStart3,854,806bp[2]End3,857,888bp[2]RNAexpressionpatternBgeeTopexpressedin duodenum jejunalmucosa Bodyofpancreas anteriorpituitary bodyofstomach kidney fundus rightuterinetube Stromalcellofendometrium endothelialcellMorereferenceexpressiondataBioGPSMorereferenceexpressiondataGeneontologyMolecularfunction nucleaseactivity endonucleaseactivity deoxyribonucleaseIactivity actinbinding GO:0001948proteinbinding hydrolaseactivity deoxyribonucleaseactivity DNAbinding Cellularcomponent extracellularregion extracellularexosome nuclearenvelope nucleus Biologicalprocess DNAcatabolicprocess nucleicacidphosphodiesterbondhydrolysis apoptoticprocess DNAcatabolicprocess,endonucleolytic neutrophilactivationinvolvedinimmuneresponse regulationofacuteinflammatoryresponse regulationofneutrophilmediatedcytotoxicity Sources:Amigo/QuickGOOrthologsSpeciesHumanMouseEntrez177313419EnsemblENSG00000213918ENSMUSG00000005980UniProtP24855P49183RefSeq(mRNA)NM_005223NM_001351825NM_001387135NM_001387139NM_001387140NM_001387141NM_010061NM_001357143RefSeq(protein)NP_005214NP_001338754NP_034191NP_001344072Location(UCSC)Chr16:3.61–3.68MbChr16:3.85–3.86MbPubMedsearch[3][4]WikidataView/EditHumanView/EditMouse DeoxyribonucleaseI(usuallycalledDNaseI),isanendonucleaseoftheDNasefamilycodedbythehumangeneDNASE1.[5] DNaseIisanucleasethatcleavesDNApreferentiallyatphosphodiesterlinkagesadjacenttoapyrimidinenucleotide,yielding5'-phosphate-terminatedpolynucleotideswithafreehydroxylgrouponposition3',onaverageproducingtetranucleotides.Itactsonsingle-strandedDNA,double-strandedDNA,andchromatin.Inadditiontoitsroleasawaste-managementendonuclease,ithasbeensuggestedtobeoneofthedeoxyribonucleasesresponsibleforDNAfragmentationduringapoptosis.[6] DNaseIbindstothecytoskeletalproteinactin.Itbindsactinmonomerswithveryhigh(sub-nanomolar)affinityandactinpolymerswithloweraffinity.Thefunctionofthisinteractionisunclear.However,sinceactin-boundDNaseIisenzymaticallyinactive,theDNase-actincomplexmightbeastorageformofDNaseIthatpreventsdamageofthegeneticinformation.ThisproteinisstoredinthezymogengranulesofthenuclearenvelopeandfunctionsbycleavingDNAinanendonucleolyticmanner. AtleastsixautosomalcodominantallelesofthegeneDNASE1havebeencharacterized,DNASE1*1throughDNASE1*6,andthesequenceofDNASE1*2representedinthisrecord.Mutationsinthisgene,aswellasfactorinactivatingitsenzymeproduct,havebeenassociatedwithsystemiclupuserythematosus(SLE),anautoimmunedisease.[7][8]ArecombinantformofthisproteinisusedtotreatoneofthesymptomsofcysticfibrosisbyhydrolyzingtheextracellularDNAinsputumandreducingitsviscosity.[9]Alternatetranscriptionalsplicevariantsofthisgenehavebeenobservedbuthavenotbeenthoroughlycharacterized.[5] Ingenomics[edit] Ingenomics,DNaseIhypersensitivesitesarethoughttobecharacterizedbyopen,accessiblechromatin;therefore,aDNaseIsensitivityassayisawidelyusedmethodologyingenomicsforidentifyingwhichregionsofthegenomearelikelytocontainactivegenes[10] DNaseISequenceSpecificity[edit] IthasbeenrecentlyreportedthatDNaseIshowssomelevelsofsequencespecificitythatmaydependonexperimentalconditions.[11]Incontrasttootherenzymeswhichhavehighsubstratespecificity,DNaseIcertainlydoesnotcleavewithanabsolutesequencespecificity.However,cleavageatsitesthatcontainCorGattheir3'endislessefficient. References[edit] ^abcGRCh38:Ensemblrelease89:ENSG00000213918-Ensembl,May2017 ^abcGRCm38:Ensemblrelease89:ENSMUSG00000005980-Ensembl,May2017 ^"HumanPubMedReference:".NationalCenterforBiotechnologyInformation,U.S.NationalLibraryofMedicine. ^"MousePubMedReference:".NationalCenterforBiotechnologyInformation,U.S.NationalLibraryofMedicine. ^ab"EntrezGene:DNASE1deoxyribonucleaseI". ^Samejima,K.&Earnshaw,W.C.(2005)."Trashingthegenome:theroleofnucleasesduringapoptosis".NatRevMolCellBiol.6(9):677–88.doi:10.1038/nrm1715.PMID 16103871.S2CID 13948545. ^HakkimA,FürnrohrBG,AmannK,LaubeB,AbedUA,BrinkmannV,HerrmannM,VollRE,ZychlinskyA(2010)."Impairmentofneutrophilextracellulartrapdegradationisassociatedwithlupusnephritis".ProcNatlAcadSciUSA.107(21):9813–8.Bibcode:2010PNAS..107.9813H.doi:10.1073/pnas.0909927107.PMC 2906830.PMID 20439745. ^YasutomoK,HoriuchiT,KagamiS,et al.(2001)."MutationofDNASE1inpeoplewithsystemiclupuserythematosus".Nat.Genet.28(4):313–4.doi:10.1038/91070.PMID 11479590.S2CID 21277651. ^ShakS,CaponDJ,HellmissR,et al.(1991)."RecombinanthumanDNaseIreducestheviscosityofcysticfibrosissputum".Proc.Natl.Acad.Sci.U.S.A.87(23):9188–92.doi:10.1073/pnas.87.23.9188.PMC 55129.PMID 2251263. ^BoyleAP,DavisS,ShulhaHP,MeltzerP,MarguliesEH,WengZ,FureyTS,CrawfordGE(2008)."High-resolutionmappingandcharacterizationofopenchromatinacrossthegenome".Cell.132(2):311–322.doi:10.1016/j.cell.2007.12.014.PMC 2669738.PMID 18243105. ^Koohy,Hashem;Down,ThomasA.;Hubbard,TimJ.;Mariño-Ramírez,Leonardo(26July2013)."ChromatinAccessibilityDataSetsShowBiasDuetoSequenceSpecificityoftheDNaseIEnzyme".PLOSONE.8(7):e69853.Bibcode:2013PLoSO...869853K.doi:10.1371/journal.pone.0069853.PMC 3724795.PMID 23922824. Furtherreading[edit] LachmannPJ(2003)."Lupusanddesoxyribonuclease".Lupus.12(3):202–6.doi:10.1191/0961203303lu357xx.PMID 12708782.S2CID 43058677. YasudaT,AwazuS,SatoW,et al.(1991)."Humangeneticallypolymorphicdeoxyribonuclease:purification,characterization,andmultiplicityofurinedeoxyribonucleaseI".J.Biochem.108(3):393–8.doi:10.1093/oxfordjournals.jbchem.a123212.PMID 2277032. KishiK,YasudaT,IkeharaY,et al.(1990)."HumanserumdeoxyribonucleaseI(DNaseI)polymorphism:patternsimilaritiesamongisozymesfromserum,urine,kidney,liver,andpancreas".Am.J.Hum.Genet.47(1):121–6.PMC 1683738.PMID 2349940. KabschW,MannherzHG,SuckD,et al.(1990)."Atomicstructureoftheactin:DNaseIcomplex".Nature.347(6288):37–44.Bibcode:1990Natur.347...37K.doi:10.1038/347037a0.PMID 2395459.S2CID 925337. KishiK,YasudaT,AwazuS,MizutaK(1989)."GeneticpolymorphismofhumanurinedeoxyribonucleaseI".Hum.Genet.81(3):295–7.doi:10.1007/BF00279009.PMID 2921043.S2CID 20420713. RosenstreichDL,TuJH,KinkadePR,et al.(1988)."Ahumanurine-derivedinterleukin1inhibitor.HomologywithdeoxyribonucleaseI".J.Exp.Med.168(5):1767–79.doi:10.1084/jem.168.5.1767.PMC 2189114.PMID 3263467. YasudaT,NadanoD,TakeshitaH,et al.(1995)."MolecularanalysisofthethirdalleleofhumandeoxyribonucleaseIpolymorphism".Ann.Hum.Genet.59(Pt2):139–47.doi:10.1111/j.1469-1809.1995.tb00737.x.PMID 7625762.S2CID 43729378. YasudaT,KishiK,YanagawaY,YoshidaA(1995)."StructureofthehumandeoxyribonucleaseI(DNaseI)gene:identificationofthenucleotidesubstitutionthatgeneratesitsclassicalgeneticpolymorphism".Ann.Hum.Genet.59(Pt1):1–15.doi:10.1111/j.1469-1809.1995.tb01601.x.PMID 7762978.S2CID 23914004. YasudaT,NadanoD,IidaR,et al.(1995)."ChromosomalassignmentofthehumandeoxyribonucleaseIgene,DNASE1(DNL1),toband16p13.3usingthepolymerasechainreaction".Cytogenet.CellGenet.70(3–4):221–3.doi:10.1159/000134038.PMID 7789176. YasudaT,NadanoD,TakeshitaH,et al.(1995)."ThemolecularbasisforgeneticpolymorphismofhumandeoxyribonucleaseI:identificationofthenucleotidesubstitutionthatgeneratesthefourthallele".FEBSLett.359(2–3):211–4.doi:10.1016/0014-5793(95)00037-A.PMID 7867802.S2CID 21461181. YasudaT,NadanoD,TenjoE,et al.(1996)."GenotypingofhumandeoxyribonucleaseIpolymorphismbythepolymerasechainreaction".Electrophoresis.16(10):1889–93.doi:10.1002/elps.11501601310.PMID 8586059.S2CID 35528773. IidaR,YasudaT,TakeshitaH,et al.(1996)."IdentificationofthenucleotidesubstitutionthatgeneratesthefourthpolymorphicsiteinhumandeoxyribonucleaseI(DNaseI)".Hum.Genet.98(4):415–8.doi:10.1007/s004390050231.PMID 8792814.S2CID 6393565. IidaR,YasudaT,AoyamaM,et al.(1998)."ThefifthalleleofthehumandeoxyribonucleaseI(DNaseI)polymorphism".Electrophoresis.18(11):1936–9.doi:10.1002/elps.1150181108.PMID 9420147.S2CID 8969865. YasudaT,TakeshitaH,IidaR,et al.(1999)."Anewallele,DNASE1*6,ofhumandeoxyribonucleaseIpolymorphismencodesanArgtoCyssubstitutionresponsibleforitsinstability".Biochem.Biophys.Res.Commun.260(1):280–3.doi:10.1006/bbrc.1999.0900.PMID 10381379. OliveriM,DagaA,CantoniC,et al.(2001)."DNaseImediatesinternucleosomalDNAdegradationinhumancellsundergoingdrug-inducedapoptosis".Eur.J.Immunol.31(3):743–51.doi:10.1002/1521-4141(200103)31:3<743::AID-IMMU743>3.0.CO;2-9.PMID 11241278. OtterbeinLR,GraceffaP,DominguezR(2001)."ThecrystalstructureofuncomplexedactinintheADPstate".Science.293(5530):708–11.doi:10.1126/science.1059700.PMID 11474115.S2CID 12030018. YasutomoK,HoriuchiT,KagamiS,et al.(2001)."MutationofDNASE1inpeoplewithsystemiclupuserythematosus".Nat.Genet.28(4):313–4.doi:10.1038/91070.PMID 11479590.S2CID 21277651. BallweberE,GallaM,AktoriesK,et al.(2001)."InteractionofADP-ribosylatedactinwithactinbindingproteins".FEBSLett.508(1):131–5.doi:10.1016/S0014-5793(01)03040-X.PMID 11707283.S2CID 7241702. TsutsumiS,KanekoY,AsaoT,et al.(2002)."DNaseIispresentinthechiefcellsofhumanandratstomachs".Histochem.J.33(9–10):531–5.doi:10.1023/A:1014999624430.PMID 12005024.S2CID 10324827. Externallinks[edit] deoxyribonuclease+IattheUSNationalLibraryofMedicineMedicalSubjectHeadings(MeSH) vteHydrolase:esterases(EC3.1)3.1.1:Carboxylicesterhydrolases Cholinesterase Acetylcholinesterase Butyrylcholinesterase Pectinesterase 6-phosphogluconolactonase PAFacetylhydrolase Lipase Bilesalt-dependent Gastric/Lingual Pancreatic Lysosomal Hormone-sensitive Endothelial Hepatic Lipoprotein Monoacylglycerol Diacylglycerol Phospholipase A1 A2 B Cutinase PETase 3.1.2:Thioesterase Palmitoylproteinthioesterase Ubiquitincarboxy-terminalhydrolaseL1 4-hydroxybenzoyl-CoAthioesterase 3.1.3:Phosphatase Alkalinephosphatase ALPI ALPL ALPP Acidphosphatase(Prostatic)/Tartrate-resistantacidphosphatase/Purpleacidphosphatases Nucleotidase Glucose6-phosphatase Fructose1,6-bisphosphatase Calcineurin Proteinphosphatase PP2A OCRL Pyruvatedehydrogenasephosphatase Fructose6-P,2-kinase:fructose2,6-bisphosphatase PTEN Phytase Beta-propellerphytase Inositol-phosphatephosphatase IMPA1,IMPA2,IMPA3 Proteinphosphatase:Proteintyrosinephosphatase Proteinserine/threoninephosphatase Dual-specificityphosphatase 3.1.4:Phosphodiesterase Autotaxin Phospholipase C D Sphingomyelinphosphodiesterase 1 PDE1 PDE2 PDE3 PDE4A/PDE4B PDE5 Lecithinase(Clostridiumperfringensalphatoxin) Cyclicnucleotidephosphodiesterase 3.1.6:Sulfatase arylsulfatase ArylsulfataseA ArylsulfataseB ArylsulfataseE Steroidsulfatase Galactosamine-6sulfatase Iduronate-2-sulfatase N-acetylglucosamine-6-sulfatase Nuclease(includesdeoxyribonucleaseandribonuclease)3.1.11-16:ExonucleaseExodeoxyribonuclease RecBCD Exoribonuclease Oligonucleotidase 3.1.21-31:EndonucleaseEndodeoxyribonuclease DeoxyribonucleaseI DeoxyribonucleaseII DeoxyribonucleaseIV Restrictionenzyme;see{{Restrictionenzyme}} UvrABCendonuclease Endoribonuclease RNaseIII RNaseH 1 2A 2B 2C RNaseP RNaseA RNaseZ RNaseE 1 2 3 4/5 RNaseT1 RNA-inducedsilencingcomplex eitherdeoxy-orribo-    NucleaseS1 Mungbeannuclease Serratiamarcescensnuclease Micrococcalnuclease vteEnzymesActivity Activesite Bindingsite Catalytictriad Oxyanionhole Enzymepromiscuity Diffusion-limitedenzyme Coenzyme Cofactor Enzymecatalysis Regulation Allostericregulation Cooperativity Enzymeinhibitor Enzymeactivator Classification ECnumber Enzymesuperfamily Enzymefamily Listofenzymes Kinetics Enzymekinetics Eadie–Hofsteediagram Hanes–Woolfplot Lineweaver–Burkplot Michaelis–Mentenkinetics Types EC1Oxidoreductases(list) EC2Transferases(list) EC3Hydrolases(list) EC4Lyases(list) EC5Isomerases(list) EC6Ligases(list) EC7Translocases(list) Portal: Biology Retrievedfrom"https://en.wikipedia.org/w/index.php?title=Deoxyribonuclease_I&oldid=1040845270" Categories:Genesonhumanchromosome16EC3.1.21 Navigationmenu Personaltools NotloggedinTalkContributionsCreateaccountLogin Namespaces ArticleTalk English Views ReadEditViewhistory More Search Navigation MainpageContentsCurrenteventsRandomarticleAboutWikipediaContactusDonate Contribute HelpLearntoeditCommunityportalRecentchangesUploadfile Tools WhatlinkshereRelatedchangesUploadfileSpecialpagesPermanentlinkPageinformationCitethispageWikidataitem Print/export DownloadasPDFPrintableversion Languages CymraegPolskiРусскийСрпски/srpskiSrpskohrvatski/српскохрватскиТатарча/tatarçaTürkçeУкраїнська Editlinks