Rag1 Knockout/Transgenic OT-I T Cell Receptor - Taconic ...

The Rag1 Knockout/Transgenic OT-I T Cell Receptor line is homozygous for a transgene that encodes a T cell receptor specific for chicken ovalbumin.. 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Model# Genotype Nomenclature 4175-FB6.129S7-Rag1tm1MomTg(TcraTcrb)1100MjbN9+N1 4175-MB6.129S7-Rag1tm1MomTg(TcraTcrb)1100MjbN9+N1 ModelDescription TheRag1Knockout/TransgenicOT-ITCellReceptorlineishomozygousforatransgenethatencodesaTcellreceptorspecificforchickenovalbumin257-264,presentedbytheMHCclassImoleculeH-2Kb.ItisalsodeficientintheRag1gene(recombinasereactivatinggene1)andthereforedoesnotdevelopmatureTorBcellsexpressingendogenousreceptors.VirtuallyallperipheralCD8+cellsexpressthetransgene.ThesemiceareusefulasasourceofhomogeneousdonorCD8+TcellsforinvivoadoptivetransferstudiestoinvestigateTcelldevelopment,activation,memory,andtolerance.TheycanbeusedinconjunctionwithCD4+TcellsfromtheRag2Knockout/TransgenicOT-IITCellReceptorline,whicharespecificforchickenovalbumin323-339,presentedbytheMHCclassIImoleculeH-2I-Ab,asapairofhelperandCTLTcellsreactiveagainstthesameantigen. GeneticBackground:C57BL/6BackgroundOrigin:TheOT-ImicroinjectedmodelwasdevelopedbyDr.FrancisCarboneofMonashMedicalSchoolandDr.MichaelBevanoftheUniversityofWashington.ThemodelwasgeneratedbyinjectingtherearrangedTcellreceptorVα2-Jα26(underthecontrolofthealphachainpromoterandenhancer)andVβ5-Dβ2-Jβ2.6(underthecontroloftheMHCclassIpromoterandtheIgheavychainenhancer)intoB6xB6.C-H2bm1blastocysts.FoundersweresubsequentlybredtoC57BL/6Jmicefor9generations.ThelinewaslatertransferredtoNIAIDfromDr.KristinHogquistoftheUniversityofMinnesotaandrederivedbymatingOT-IsourcemalestoC57BL/6NAifemales.ThetransgenicmicewerecrossedtwicetoahomozygousC57BL/6J-Rag1tm1Momknockoutmousewhichhadbeenbackcrossed10generationstoB6/J(MombaertsP,Cell1992;68:869-877),andRag1-/-offspringwereselected.ThesemicewerethenintercrossedandOT-ITCRpositivemicehomozygousforthetransgenewereidentifiedbytestmatingthemtowildtypepartnersandselectinganimalsthatonlygaverisetotransgenepositiveoffspring.Availability:Thismodelisunavailable.Color:BlackSpecies:MouseInitialPublication:HogquistKA,JamesonSC,HeathWR,HowardJL,BevanMJandCarboneFR:TCellreceptorantagonistpeptidesinducepositiveselection;Cell1994:76:17-27 LiM,DaveyGM,SutherlandRM,KurtsC,LewAM,HirstC,CarboneFR,HeathWR.;Cell-associatedovalbuminiscross-presentedmuchmoreefficientlythansolubleovalbumininvivo.JImmunol2001166:6099-6103. MombaertsP,IacominiJ,JohnsonRS,HerrupK,TonegawaSandPapaioannouVF:RAG-IdeficientmicehavenomatureBandTcells.Cell199268(5):869 Findyourmodel GEMsTaconicTransgenicModels™EmergingModelsCryopreservedModelsStandardStrainsandStocksStandardImmunodeficientsDietInducedMiceHumanizedImmuneSystemMiceTherapeuticAreas&Solutions2022PricingGuide 2022PricingGuideCreateyourcustomPricingGuide RelatedAnimalModels Rag2/OT-I RelatedPublications Tcellreceptorantagonistpeptidesinducepositiveselection.Cell-associatedovalbuminiscross-presentedmuchmoreefficientlythansolubleovalbumininvivo.SearchallRag1Knockout/TransgenicOT-ITCellReceptorpublications CustomerService NeedadditionalhelpfindingandorderingTaconicproductsandservices? CONTACTUS 888-TACONIC(822-6642) +4570230405(Europe) Fax:+1(518)697-3910 Close Welcome!Tellusalittleaboutyourself Hereweshowthechatwindow.