人類在生理週期中紅血球內穀胱甘肽和過氧化氫酶的表現
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女性依自然月經週期分兩組:經期後第10-20天為A組(16人),是動情激素高峰時期; ... 因此,我們推論紅血球內抗氧化物質穀胱甘肽及過氧化氫酶含量與動情激素表現量有關。
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本論文永久網址: 複製永久網址Twitter研究生:蘇琬玲研究生(外文):Wan-Ling論文名稱:人類在生理週期中紅血球內穀胱甘肽和過氧化氫酶的表現論文名稱(外文):Theexpressionsofhumanglutathioneandcatalaseinerythrocyteduringthemenstrualcycle指導教授:劉哲育指導教授(外文):Jer-YuhLiu學位類別:碩士校院名稱:中山醫學大學系所名稱:生化暨生物科技研究所學門:生命科學學門學類:生物科技學類論文種類:學術論文論文出版年:2008畢業學年度:96語文別:中文論文頁數:50相關次數:
被引用:0點閱:187評分:下載:0書目收藏:0
本實驗主要是研究動情激素與紅血球內抗氧化物質如穀胱甘肽(glutathione;GSH)和過氧化氫酶(catalase)的相關性。
採集總人數為59人,女性43人,男性16人。
女性依自然月經週期分兩組:經期後第10-20天為A組(16人),是動情激素高峰時期;第0-9天及21-30天為B組(27人),是動情激素低量期。
A組穀胱甘肽的含量(4.4±2.3μg/mg)較B組(3.2±1.8μg/mg)為顯著高(P<0.05)。
而且A組過氧化氫酶的活性(210±72IU/mg)也較B組(168±62IU/mg)為顯著高(P<0.05)。
同時動情激素變化A組(184±106pg/mL)也是較B組(105±56pg/mL)為顯著高(P<0.01)。
若依動情激素平均值(134pg/mL)為界線分兩組,平均值以上為C組(13人),平均值以下為D組(29人)。
穀胱甘肽含量C組(4.0±1.6μg/mg)與D組(3.5±2.2μg/mg)無顯著差異。
然而過氧化氫酶的活性C組(217±78IU/mg)較D組(167±60IU/mg)為顯著高(P<0.05)。
另外男性與女性各組並無顯著差異。
因此,我們推論紅血球內抗氧化物質穀胱甘肽及過氧化氫酶含量與動情激素表現量有關。
Thepurposeofthisstudyistoinvestigatethecorrelationbetweenestrogenandantioxidantssuchas(GlutathioneGSH,catalase)intheerythrocytes.Thetotalnumberpopulationofthisstudywasconsistantedof59healthypersons(43femaleand16male).Thefemalegroupwasdividedinto2groupsaccordingmenstrualcycle:groupAconsistedof16female(10-20daysaftermenstrualcycle)isinthestageofhigherestrogenlevel;groupBconsistedof27females(1-9and21-30daysaftermenstrualcycle)isinthelowerestrogenlevel.TheresultsshowedthattheglutathioneGSHlevelingroupA(4.4±2.3μg/mg)wassignificantlyhigherthanthatingroupB(3.2±1.8μg/mg)(P<0.05).howeverthecatalaselevelingroupA(210±72IU/mg)wasalsosignificantlyhigherthanthatingroupB(168±62IU/mg)(P<0.05).TheestrogenlevelingroupA(184±106pg/mL)ishigherthanthatingroupB(105±56pg/mL)(P<0.01).Accordingtotheaverageestrogen134pg/mLthehigherestrogenlevelgroupC(n=13)andlowerestrogenlevelgroupD(n=29).Thesamplesweredividedistotwogroups.ThereisnodifferenceofGlutathionelevelbetweengroupC(4.0±1.6μg/mg)andgroupD(3.5±2.2μg/mg).HoweverthereissignificantdifferenceofcatalaselevelbetweengroupC(217±78IU/mg)andgroupD(167±60IU/mg)(P<0.05).Nodifferenceofantioxidantswasobservedinbetweenmaleandfemale.Thuswesuggestedthattheexpressionofantioxidants(Glutathioneandcatalase)inerythrocyteiscorrelatedwiththelevelofestrogen.
目錄壹、中文摘要……………………………………………………………3貳、英文摘要…………………………………………………………4參、縮寫表…………………………………………………………….5肆、前言…………………………………………………………………7伍、實驗動機……………………………………………………………16陸、實驗方法與材料……………………………………………………17柒、實驗結果…………………………………………………………23捌、討論……………………………………………………………….26玖、參考文獻……………………………………………………………30拾、圖表…………………………………………………………………43
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