A tool for telling apart C57BL/6J versus C57BL/6N substrains

The Jackson Laboratory's C57BL/6 Substrain Characterization Panel can determine the relative contributions of C57BL/6J and C57BL/6N to a B6 ... Research&Faculty  Overview Locations JAXMammalianGenetics-Maine JAXGenomicMedicine-Connecticut A-ZFacultyListing A-ZResearchLabs DataScienceatJAX ToolsandResources ResearchCenters Careers Education&Learning  Education&LearningHome OnlineLearning Courses InPerson&Virtual OnlineMicroLessonsandMiniCourses BioinformaticsTrainingProgram Clinicians ClinicalTopics CourseOfferings CancerGeneticClinicalEducation PrecisionMedicineforYourPractice Postbacc,Ph.D.&Postdocs CooperativePh.D.Training PostdoctoralAssociates PostbaccFellows PostbaccalaureateResearchers Students&Teachers MaineStateScienceFair SummerStudentProgram AcademicYearFellowships TeacherProfessionalDevelopment GeneticsLearningResources CollegeScholarship Jax®Mice&Services  InnovativeResearch BreakthroughLab ResearchResiliency FindMice&Biospecimens WhyJAXMice SearchJAXMice OrderJAXMice Biospecimens ServicesforMice CustomModelGeneration ColonyManagement Surgical&PreconditioningServices InVivoPharmacology Oncology PDXModels Humanized Neurobiology Immunology AntibodyEvaluation SafetyandEfficacyStudies DiseaseArea Cardiovascular Immunology MetabolicDiseases Neurobiology Oncology RareDiseases CustomerResources CustomerService TechnicalHelp ResourceCenter VirtualEvents DiscoverJAX Webinars PersonalizedMedicine  WhatisPersonalizedMedicine? Geneticsvs.genomics Ethicalconsiderations Personalizedmedicineandyou WhatisCRISPR? MiceinBiomedicalResearch AreasofResearch Addiction Aging Alzheimer's&OtherDementias Cancer Diabetes Microbiome Rarediseases Vision ClinicalKnowledgebase MaineCancerGenomicsInitiative News  JAXNews JAXBlog MinutetoUnderstanding Subscribe PressRoom TheSearchMagazine ExplorebyTopic AboutUs  FastFacts OurPeople&Culture OurValues Diversity,Equity&Inclusion FacesofJAX OurLeaders WorkWithUs OurCampuses&Communities OurImpact CollaborationatJAX OurMedicalImpact OurEconomicImpact OurCommunityImpact OurEnvironmentalImpact OurHistory Careers ContactUs Give JAXHome NewsandInsights eNews April04,2013 AtoolfortellingapartC57BL/6JversusC57BL/6Nsubstrains IfyouneedtoknowwhetheryourmousehasaC57BL/6J(B6J,000664),C57BL/6N(B6N,005304),ormixedB6J;B6Nbackground,TheJacksonLaboratory’s(JAX’s)newC57BL/6SubstrainCharacterizationPanel maybejustthetoolyouneed. Doesitreallymatter? DoesitmakeadifferenceifyourmousehasaB6J,B6N,ormixedB6J;B6Nbackground?Ifyouransweris“No,”considerthefollowing: C57BL/6JhastheNntmutation,whichmodulatesmetabolism(Freemanetal.2006;Shimomuraetal.2009)andimmuneresponse(Ripolletal.2012). C57BL/6JandC57BL/6Nvaryintheirfearresponses(Radulovicetal.1998;Stiedletal.1999)andhowanestheticsaffecttheircardiacfunctions(Rothetal.2002). C57BL/6NmiceandembryonicstemcellsderivedfromthemharbortheRd8mutationoftheCrb1gene,whichcausesdevelopmentaldefectsinphotoreceptorsegments(Mehalowetal.2003;Mattapalliletal.2012). MiceproducedbytheKnockoutMouseProject(KOMP)areonaB6Nbackground,whichmeansthatifyoubreedthemtoyourB6colony,youmaybeproducingoffspringwithamixedB6J;B6Ngeneticbackground. Whenyoureceivestrainsfromothersources,keepingthemutationonsimilarstrainbackgroundreducesthechancethatyouwillalterorloseyourstrain’sphenotypeduetobackgroundmodifiergenes. Onceyouknowthebackgroundofyourmousestrain,youcanbetteridentifytherightcontrolstrain,whichbackgroundtoselectforbackcrossing,andwhatstrain-specificphenotypesyoushouldbeawareof. Howyoucanusethe C57BL/6SubstrainCharacterizationPanel JAX’snewC57BL/6SubstrainCharacterizationPanel coversallautosomesandtheXchromosome.Thegenome-widespacingaverages15.4+/-7.3Mb–equivalenttoapproximately8centiMorgans. NumberofSNPmarkersperchromosomeintheC57BL/6SubstrainCharacterizationPanel: Chr# 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 NoofSNPs 12 10 9 8 7 8 9 8 7 7 7 7 7 7 6 6 5 6 4 10 Youcansendsamplesfromyourbreedingprojectstousfortestingorwecanperformtestinginconjunctionwithanyprojectyouchoosetooutsourcetous.JAXcanperformgenomescanningasastand-aloneserviceorintegrateitwithcryopreservation,rederivationorbreedingservicesforyou. JAXdistributesbothC57BL/6J(000664)andC57BL/6NJ(005304)mousestrains FormoreinformationabouttheC57BL/6SubstrainCharacterizationPanel,contactTechnicalInformationServicesat1-800-422-6423,1-207-288-5845,[email protected]. Topics ModelDevelopment ResearchHighlight October27,2020 Notalleggsinonebasket:geneticalternativesregulatingmeioticrecombination GeneticTools ModelDevelopment TechCorner September25,2020 CharlesLeeappearsonCBIABizCast COVID-19 InfectiousDisease ModelDevelopment ResearchHighlight April02,2019 Thehowandwhyofresearchwithdiversitymousestrainsandpopulations ModelDevelopment PressRelease December27,2018 NewAlzheimer'smousemodelsshowhowhumandiversityaffectsdiseaseonset GeneticTools ModelDevelopment NeurodegenerativeDisease Neuroscience Aging YouMayAlsoBeInterestedin Oct 03-06 Workshop ColonyManagement&Biomethods Jun 27-30 Workshop WorkshoponSurgicalTechniquesintheLaboratoryMouse(inParis,France) ModelDevelopment, GeneticTools Oct 10-13 ShortCourse TheShortCourseontheApplicationofMachineLearningforAutomatedQuantificationofBehavior LungsRespiratory, ModelDevelopment Jun 21-23 Workshop WorkshoponStereotaxicSurgeryintheLaboratoryMouse(inParis,France) ModelDevelopment, Neuroscience, NeurodevelopmentalDefects May 03-05 StereotaxicSurgeryintheLaboratoryMouse Aug 16-18 StereotaxicSurgeryintheLaboratoryMouse Apr 26-30 Workshop PrinciplesandTechniquesforImprovingPreclinicaltoClinicalTranslationinAlzheimer'sDiseaseResearch Oct 17-20 Conference ImpactsofSleepandCircadianBiologyonAlzheimer'sDiseaseandAging LungsRespiratory, ModelDevelopment