Comparison between V3V4 and full-length sequencing of 16S ...
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341F, CCTACGGGNGGCWGCAG. 805R, GACTACHVGGGTATCTAATCC. PacBio primers targeting full-length 16S rRNA of Bacteria. 27F, AGRGTTYGATYMTGGCTCAG. Skiptocontent ComparisonbetweenV3V4andfull-lengthsequencingof16SrRNAgenes JonJongsikChun,Ph.D. May15,2017 SubscribeToOurNewsletter Getupdatesandlearnfromthebest Email Send Microbiometaxonomicprofiling(MTP)isamethodtoelucidatetaxonomiccompositionofthecomplexmicrobialcommunity.ThecurrentlyavailableandwidelyusedNGSplatformsareIlluminaMiSeq(pair-end250bp)targetingV3V4regionandPacificBiosciences(PacBio)targetingfull-length.Here,wegivestatisticsonlengthsofamplicons. VariousPCRprimersthatarefrequentlyusedtopartsof16SrRNAgenearegivenhere. TheamplificationofprimersusedbyChunLabare: MiSeqprimerstargetingV3V4regionofBacteria 341F CCTACGGGNGGCWGCAG 805R GACTACHVGGGTATCTAATCC PacBioprimerstargetingfull-length16SrRNA ofBacteria 27F AGRGTTYGATYMTGGCTCAG 1492R RGYTACCTTGTTACGACTT Statisticsofampliconlengths ThelengthswerecalculatedfromreferencesequencesincludedinEzBioCloud16Sdatabases(Yoonetal.2017).Thevaluesarewithoutprimers. LastUpdatedMar.20,2017(JC) SubscribeToOurNewsletter Getupdatesandlearnfromthebest Lastname Email Send MoreToExplore Pan-genomeorthologousgrouptable Thistablecontainsinformationaboutpan-genomeorthologousgroups(POGs)inadataset.For generating“pan-genome”,pleasevisithere.Forpan-genomeorthologousgroups,pleasevisithere. ChunLabInc. 05/15/2017 Howtofindpublicmicrobiomedatabasedon16SrRNAgene Majorityofmicrobiometaxonomicprofiledataarebasedon16SrRNAgenetargettingbacteria.Inthisdocument,Iwillexplainhowtosearchandlocate ChunLabInc. 09/07/2019 ShareThisPost Shareonfacebook Shareonlinkedin Shareontwitter Shareonemail Newsletter PrevPreviousHowtouseEzBioCloud16SdatabasewithMOTHUR NextHowtouseEzBioCloud16SdatabasewithQIIMENext PoweredbyPrecision,DrivenbyQuality Sitemap UserGuide FAQ Contactinfo ChunLab,Inc Twitter Linkedin Envelope [email protected] EzBiome,Inc Twitter Linkedin Envelope [email protected] Address ChunLab,Inc ChunLabTower,34Samseong-ro85-gilGangnam-guSeoulRepublicofKorea(ZIP06194) EzBiome,Inc 704QuinceOrchardRoad,Suite250,Gaithersburg,Maryland,20878,UnitedStatesofAmerica Familysites www.ezbiocloud.net www.smilebiome.com www.chunlab.com HaveaQuestion?Let'shaveachat? We'reheretoansweranyquestionyoumighthave FullName Email TestArea Send Orbookameeting HaveaQuestion?Let'shaveachat? We'reheretoansweranyquestionyoumighthave FullName Email TestArea Send Orbookameeting Stayuptodate Keepupwithourlatestdevelopments FirstName LastName Email Send
延伸文章資訊
- 1Decontamination of 16S rRNA gene amplicon sequence ...
... and 0.5 μl each of 10 μM forward primer 341 F 5'-CCTACGGGNGGCWGCAG-3' and reverse primer 805R...
- 2Microbial Community Composition and Diversity via 16S rRNA ...
... sequencing by complementing both Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACHVGGGTATC...
- 3Evaluation of PCR conditions for characterizing bacterial ...
... and V4 hypervariable regions of the 16S rRNA genes using primers 341F (5′-CCTACGGGNGGCWGCAG-3...
- 4Comparison between V3V4 and full-length sequencing of 16S ...
341F, CCTACGGGNGGCWGCAG. 805R, GACTACHVGGGTATCTAATCC. PacBio primers targeting full-length 16S rR...
- 5Questions about V3–V4 primers for 16S rRNA amplicon ...
That paper reports primers called Bakt_341F (CCTACGGGNGGCWGCAG) and Bakt_805R (GACTACHVGGGTATCTAA...
