於小鼠動情週期不同期別進行超級排卵處理對卵子與胚銘印基因 ...

小鼠動情週期觀察及陰道抹片檢查。

實驗動物飼養管理實習，第七章。

屏東科技大學，台灣。

Abdalla, H., Y. Yoshizawa, and S. Hochi. 2009. Skipnavigation 中文 English 中興大學機構典藏NCHUInstitutionalRepository 中興大學機構典藏DSpace系統致力於保存各式數位資料（如：文字、圖片、PDF）並使其易於取用。

NCHUInstitutionRepository 農業暨自然資源學院 動物科學系 請用此HandleURI來引用此文件： http://hdl.handle.net/11455/25591 標題: 於小鼠動情週期不同期別進行超級排卵處理對卵子與胚銘印基因H19與Snrpn維持之影響Theimpactofsuperovulationadministrationatdifferentestrouscyclestagesinmiceonthemaintenanceofimprintedgenes,H19andSnrpn 作者: 林俊廷Lin,Chun-Ting 關鍵字: 人工生殖技術;ART;超級排卵;甲基化差異區域;銘印;Superovulation;DMR;Imprinting 出版社: 動物科學系所 引用: 林俊廷。

2007。

小鼠動情週期觀察及陰道抹片檢查。

實驗動物飼養管理實習，第七章。

屏東科技大學，台灣。

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銘印基因為只表現父方或母方同源染色體上之基因，其主要調控方式為於銘印基因啟動子進行甲基化程度，此區域稱為甲基化差異區域（DifferentiallyMethylatedRegion,DMR）。

本研究之目的為探討小鼠於不同動情週期期別，包含發情前期、發情期、發情後期以及間期施以超級排卵，對銘印基因H19與Snrpn啟動子甲基化狀態之影響。

母鼠以陰道抹片確認其動情週期期別後，施以超級排卵，收集第二次減數分裂中期（metaphaseII,MII）之卵母細胞，一部分進行孤雌激活（pathenogeneticallyactivated,PA）並培養至囊胚期（blastocyststage），而有些小鼠經超級排卵處理後，配種並收集原核期（pronuclearstage）受精卵培養至囊胚（fertilizedblastocyst,FB）階段再進行分析。

增殖之H19與Snrpn片段經亞硫酸鈉處理後，進行定序並分析其甲基化狀態與銘印基因CpGisland之關聯性。

卵母細胞分析結果顯示，母方銘印並未受到超級排卵處理影響；FB組之囊胚顯示具有異常甲基化銘印位點，而於發情後期施以超級排卵之PA組囊胚，其Snrpn銘印區域甲基化有顯著之缺失。

本研究結果顯示於受精後，超級排卵可能造成母系產物異常導致銘印基因混亂，但其機制有待更進一步之研究。

Assistedreproductivetechnology(ART)isroutinelyappliedtothetreatmentsofsubfertilityincouples,researchesinanimalmodels,aswellasproductionoflivestocks.However,accumulatingevidencesindicatethatthegenerationsderivedfromARTmaysuffertheincreasedriskofintrauterinegrowthretardation,prematurebirth,lowbirthweight,orgenomicimprintingdisorders.Imprintedgenesshowpredominantorexclusivetranscriptionfromoneparentalalleleonly.MethylationoftheDNAinregionsclosetotheimprintedgenespromoteristhoughttoplayakeyroleinregulatingimprintedgeneexpressionandlossofmethylationinthesedifferentiallymethylatedregions(DMR)isassociatedwithlossofimprinting.Therefore,theaimofthisstudywastoinvestigatetheeffectsofadministrationofsuperovulationatdifferentestrouscyclestages,includingproestrus,estrus,metestrusanddiestrus,onthemethylationstatusoftheimprintedgenesH19andSnrpnDMR.Femalemiceweresuperovulatedaccordingtothevaginalsmears.MetaphaseII(MII)oocyteswerecollected.SomeoftheMIIooctyeswerepathenogeneticallyactivatedandculturedtotheblastocyststage(PA).Additionally,fertilizedpronuclearstageembryoswerealsocollectedandculturedtotheblastocyststage(FB).ThemethylationstatusofDMRonH19andSnrpnwereanalyzedbycloningandsequencingfollowingDNAbisulfitetreatment.TheresultsfromtheanalysisofoocytesdemonstratedthatthematernalimprintingacquisitionwasnotaffectedbysuperovulationinbothH19andSnrpn.However,theimprintingpatternsintheFBblastocystsshowedtheaberrantDNAmethylationintheimprintingloci,andthePAblastocystsshowedthesignificantdifferencesintheimprintinglossofSnrpnatthemetestrusgroup.AnalysisofimprintingCpGislandsindicatedthecorrelationamongthemethylationsites.Theseresultsimplythattheimprintingdisordercausedbysuperovulationmaychangethematernal-inheritedgeneproductsrequiredfortheimprintingmaintenanceafterfertilization. URI: http://hdl.handle.net/11455/25591 其他識別: U0005-2808201206510600 顯示於：動物科學系 顯示文件完整紀錄   TAIR相關文章 國立交通大學 - 探討剔除白色念珠菌CaORF19.3360、CaORF19.4965和CaORF19.6268對白色念珠菌黏附、型態變化和生長情況之影響/郭郁琳;Kuo,Yu-Lin;楊昀良;Yang,Yun-Liang GoogleScholarTM 檢查 在IR系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。