簡麗鳳 - 中興大學生命科學系

綠藻光合產氫 3.竹筍之能量替代路徑 研究主題: 1.製造zero-CO2-emission 綠藻工廠以進行光合產氫 2.小球綠藻Chlorella可表現有活性有功能之外來蛋白質 簡麗鳳[副教授] 生物能量研究室 辦公室電話： (04)22840416#609 實驗室電話： (04)22840416#608 傳  &nbsp   真 ： (04)22874740 實驗室位置： 生命科學大樓6樓606室 最高學歷 ： 英國劍橋大學生物化學博士 專長領域 ： 生物能量代謝機制之調控分析  電子信箱 ： [email protected] 實驗室網站：     學歷 經歷 研究方向 開設課程 研究室人員 學術著作 年份 標題 2014 2014指導台中一中同學中區科展特優   畢業學校 主修學門系所 學位 時期 英國劍橋大學 生物化學系 博士 1992-1996 清華大學 輻射生物研究所 碩士 1988-1990 中原大學 化學系 學士 1982-1986   服務機關 服務部門 職稱 時期 中興大學 生命科學系 副教授 2011.08-迄今 中興大學 生命科學系 助理教授 2002-2011 中興大學 植物系 助理教授 2000-2002 以色列本古里昂大學 布勞斯丁沙漠研究所 博士後研究員 1999-2000 東華大學 生物技術研究所 副研究員 1998-1999 中央研究院 植物/分子生物研究所 博士後研究員 1996-1998   研究興趣:1.能量轉換調控分析2.綠藻光合產氫3.竹筍之能量替代路徑研究主題:1.製造zero-CO2-emission綠藻工廠以進行光合產氫2.小球綠藻Chlorella可表現有活性有功能之外來蛋白質3.竹筍之能量替代路徑之調控分析:對抗環境壓力目前研究計畫1.小球藻產氫路徑之分析(中興NCHU101,102,103,104,105,106)2.蛙類啟發之仿生水下抗生物汙染附著表面塗層(中興ENBAL2020/共同計畫主持人)3.氫化酶-奈米複合材料之生物燃料電池:光催化綠藻氫能(科技部MOST110-2221-E-005-078)   學期 科目名稱 學分 開設班級 下學期 植物生理學 下學期 生物物理化學 下學期 生物物理特論 上學期 普通植物學 上學期 植物生理學 上學期 生物能量學 上學期 生物光譜學 博士班碩士班黃文翰陳嘉琪大學部研究助理兼任-王OO博士後研究畢業生:陳秀惠(博)、徐晁偉(碩)、林彥儒(碩)、徐靖雯(碩)、楊大緯(碩)、柯冠仲(碩)、謝鎮鴻(碩)、劉邦弘(碩)、郭婷婷(碩)、邱嘉源(碩)、林昕笛(碩)、劉芫攸(碩/共同指導)、陶致蓁(碩/共同指導)、陳惠萍(碩)、吳苑菁(碩)、陳孟偉(碩)、林文豪(碩)、何杰龍(碩)、林昱廷(學士畢)等   A.期刊論文  1.TsaiHC;HsiehCH;HsuCW;HsuYH;ChienLF.*2022/04CloningandOrganelleExpressionofBambooMitochondrialComplexISubunitsNad1,Nad2,Nad4,andNad5intheYeastSaccharomycescerevisiae.,InternationalJournalofMolecularSciences[IF=5.924(2020);Rank=67/295(22.7%)inBIOCHEMISTRY&MOLECULARBIOLOGY].vol.23,pp.4054.(SCI) 2.ChenTL,LinYP,ChienCH,ChenYC,YangYJ,WangWL,ChienLF,HsuehHY*2021/10FabricationofFrog-Skin-InspiredSlipperyAntibiofoulingCoatingsThroughDegradableBlockCopolymerWrinkling.,ADVANCEDFUNCTIONALMATERIALS[IF=18.808(2020),Rank=13/314(4.1%)inMaterialsScience-Multidisciplinary].vol.31,no.42,pp.2170309.(SCI) 3.ChienLF;LinWH2019/09ChangesinphotochemicalefficiencyanddifferentialinductionofsuperoxidedismutaseinresponsetocombinedstressesofchillingtemperatureandrelativelyhighirradiationintwoChlorellastrains.DOI:10.5772/intechopen.89024,IntechOpen:Microalgae-FromPhysiologytoApplication.vol.bookchapter.(其他) 4.Yang,DW;Syn,JW;Hsieh,CH;Huang,CC;Chien,LF*2019/01Geneticallyengineeredhydrogenasespromotebiophotocatalysis-mediatedH2productioninthegreenalgaChlorellasp.DT,InternationalJournalofHydrogenEnergy[IF=4.084(2018);Rank:31/103(30.1%)ofEnergy&Fuel].vol.44,no.5,pp.2533-2545.(SCI) 5.ChenYS,ChessonP,WuHW,PaoSH,LiuJW,ChienLF,YongJW,SheueCR*.2017/03Leafstructureaffectsaplant'sappearance:combinedmultiple-mechanismsintensifyremarkablefoliarvariegation.,JournalofPlantResearch[IF=1.899(2016);Rank:73/212(36.4%)ofPLANTSCIENCES].vol.130,no.2,pp.311-325.(SCI) 6.ChenHH,HuangCC,ChengEH,LeeTH,ChienLF*,LeeMS*.2017Optimaltimingofblastocystvitrificationaftertrophectodermbiopsyforpreimplantationgeneticscreening.,PLoSOne[IF=2.806(2016);Rank:15/128(11.7%)ofMULTIDISCIPLINARYSCIENCES].vol.12,no.10,pp.e0185747.(SCI) 7.Hsiu-HuiChen,Tung-ChuanYang,Tsung-HsienLee,Chun-ChiaHuang,Maw-ShengLee*,Lee-FengChien*2016/08Womanagelimitstheimprovableeffectofreducedoxygenconcentration(5%O2)onhumanembryoqualityandpregnancypotential,InternationalJournalofClinicalandExperimentalMedicine..vol.9,pp.16459-16467.(SCI) 8.LinHD,LiuBH,KuoTT,TsaiHC,FengTY,HuangCC*,ChienLF*.2013KnockdownofPsbOleadstoinductionofHydAandproductionofphotobiologicalH2inthegreenalgaChlorellasp.DT.,BioresourceTechnology.[IF=4.494(2014);Rank:13/89(14.6%)ofEnergy&Fuels].vol.143,pp.154-162.(SCI) 9.ChienLF,*,KuoTT,LiuBH,LinHD,FengTY,HuangCC*.2012/12Solar-to-bioH2productionenhancedbyhomologousoverexpressionofhydrogenaseingreenalgaChlorellasp.DT.,InternationJournalofHydrogenEnergy(IF=3.313;Rank:25/89[28.0%]/Energy&Fuels/2014).vol.37,pp.17738-17748.(SCI) 10.SheueCR*,PaoSH,ChienLF,ChessonP,PengCI.2012Naturalfoliarvariegationwithoutcosts?ThecaseofBegonia,AnnalsofBotany(London)[IF=3.654(2014);Rank:26/204(12.7%)ofPlantScience].vol.109,pp.1065-1074.(SCI) 11.WengJH*,ChienLF,JiangCY,ShihFC,ChenHY.2011Acomparisonbetweenyellow-greenandgreencultivarsoffourvegetablespeciesinpigments,ascorbate,photosynthesis,energydissipation,andphotoinhibition.,Photosynthetica(IF=1.409;Rank:99/204[48.5%]/PlantSciences/2014).vol.49,pp.361-370.(SCI) 12.ChienLF*,WuYC,ChenHP.2011MitochondrialenergymetabolisminyoungbamboorhizomesfromBambusaoldhamiiandPhyllostachysedulisduringshootingstage.,PlantPhysiol.Biochem.(IF=2.756;Rank:44/204[21.6%]/PlantSciences/2014).vol.49,pp.449-457.(SCI) 13.ShiehGJ,ChienLF*,PanRL2011Theextrinsicproteinsofoxygen-evolvingcomplexinmarinediatomCylindrothecafusiformis.,BotanicalStudies(IF=0.783;Rank:145/204[71.1%]/PlantScience/2014).vol.52,pp.161-171.(SCI) 14.ChienLF*,VonshakA.2011EnzymaticantioxidantresponsetolowtemperatureacclimationinthecyanobacteriumArthrospiraplatensis.,JournalofAppliedPhycology(IF=2.559;Rank:18/103[17.5%],Marine&Freshwater/2014).vol.23,pp.887-894.(SCI) 15.HsiaoY-Y,PanY-J,HsuS-H,HuangY-T,LiuT-H,LeeC-H,LeeC-H,LiuP-F,ChangW-C,WangY-K,ChienL-F,PanR-L*2007FunctionalrolesofarginineresiduesinmungbeanvacuolarH+-pyrophosphatase.,Biochim.Biophys.Acta-Bioenergetics(IF=5.353;Rank:11/74[14.9%]/Biophysics/2014).vol.1767,pp.965-973.(SCI) 16.HuangC-C,ChenM-W,HsiehJ-L,LinW-H,ChenP-C,ChienL-F*.2006ExpressionofmercuricreductasefromBacillusmegateriumMB1ineukaryoticmicroalgaChlorellaspDT,Appl.Microb.Biotechnol.(IF=3.337;Rank:39/163[23.9%]/Biotechnology&AppliedMicrobiology/2014).vol.72,pp.197-205.(SCI) 17.LeeSC,ChienLF*,VanRC,HsiaoYY,HongJL,PanRL.2006Radiationinactivationanalysisofthylakoidproteinkinasesystemsinlightandindarkness,Photosynthetica.(IF=1.409;Rank:99/204[48.5%]/PlantSciences/2014).vol.44,pp.116-124.(SCI) 18.KuoSY,ChienLF,VanRC,YanKH,LiuPF,ChangWC,WangJK,PanRL2005PurificationandsubunitdeterminationofinorganicH+-pyrophosphatasefromendoplasmicreticulum-enrichedvesiclesofmungbeanseedlings,PlantSci.169.pp.847-853.(SCI) 19.KuoSY,ChienLF,HsiaoYY,VanRC,YanKH,LiuPF,MaoSJ,PanRL2005Protonpumpinginorganicpyrophosphataseofendoplasmicreticulum-enrichedvesiclesfrometiolatedmmungbeanseedlings,J.PlantPhysiol.162.pp.129-138.(SCI) B.研討會論文  1.KuoTT,LinYJ,ChienLF*.2017/09Invitedspeaker:GreenandcleanbioenergyH2producedbygreenalgaChlorellasp.DTtransgenicsduringbiophotolysis.Abstract:3rdWorldCongressandExpoonGreenEnergy.28-29September2017,Berlin,Germany,3rdWorldCongressandExpoonGreenEnergy.28-29September2017,Berlin,Germany.(其他) 2.Ting-TingKuo,Yen-JuLin,Lee-FengChien*2016/08Invitedspeaker:CleanbioenergyH2photobiologicallyproducedbygreenalgalChlorellasp.DTmutantswithoverexpressedhydrogenaseorferredoxin.,6thInternationalSymposiumonEnergyChallengesandMechanics.TowardsaBigPicture..Inverness,Scotland,UK.(其他) 3.Ting-TingKuo,Yen-JuLin,Lee-FengChien*2016Invitedspeaker:CleanenergyH2photobiologicallyproducedbygreenalgalChlorellasp.DTmutantswithoverexpressedhydrogenaseorferredoxin.Abstract.,2016光合作用與植物生理研討會.Taipei,Taiwan.(其他) 4.SyuJW,YangDW,ChienLF.2014ThehydrogenproductionofhighoxygentolerancehydrogenasemutantofCholrellasp.DT.PosterAbstract:2014PlantScienceAnnualMeeting&Symposium,13-14December2014,Taipei,Taiwan. 5.LinYJ,YangDW,ChienLF2014Constructionofp121-crfd-gfpvectorforoverexpressingferredoxin1inChlorellasp.DThydrogenasemutants.PosterAbstract:2014PlantScienceAnnualMeeting&Symposium,13-14December2014,Taipei,Taiwan.. 6.KuoTT,LiuBH,LinHD,FengTY,HuangCC,ChienLF.2014OverexpressionofHydAorknockdownofPsbOleadtotheincreaseinthephotobiologicalH2productioningreenalgaChlorellasp.DT.PosterAbstract:The15thIUBMB-24thFAOBMB-TSBMBConference.21-26thOct,2014,Taipei,Taiwan. 7.KuoTT,LiuBH,LinHD,FengTY,HuangCC,ChienLF*.2014KuoTT,LiuBH,LinHD,FengTY,HuangCC,ChienLF.(2014)OverexpressionofHydAorknockdownofPsbOleadtotheincreaseinthephotobiologicalH2productioningreenalgaChlorellasp.DT.PosterAbstract.,The15thIUBMB-24thFAOBMB-TSBMBConference.21-26October2014,Taipei,Taiwan.(其他) 8.HsiehCW,ChenLF.2013/03Expressionofmitochondrial-encodedNad2andNad5subunitsofbambooPhyllostachysedulismitochondrialNADH:ubiquinoneoxidoreductaseinSaccharomycescerevisiae.PosterAbstract:The28thJointAnnualConferenceofBiomedi,The28thJointAnnualConferenceofBiomedi.Taipei,Taiwan.(其他) 9.HsuCW,ChenLF2013Expressionofmitochondrial-encodedNad2andNad5subunitsofbambooPhyllostachysedulismitochondrialNADH:ubiquinoneoxidoreductaseinSaccharomycescerevisiae.PosterAbstract:,The28thJointAnnualConferenceofBiomedicalSciences,16-17March,2013.Taipei,Taiwan. 10.YangDW,ChenLF2013GreenalgaChlorellahydrogenasemutantsthathavehighO2tolerance.PosterAbstract:The28thJointAnnualConferenceofBiomedicalSciences,16-17March,2013,Taipei,Taiwan.P726. 11.LinHD,LiuBH,KuoTT,ChienLF*.2012Invitedspeaker:(IFIB-2012BiorefineryTechnology7)KnockdownofPsbOleadstotheinductionofHydAandtheproductionofphotobiologicalH2ingreenalgaChlorellasp.DT,Abstract:IFIB-20125thInternationalConferenceonIndustrialBioprocessinTaiwan7-10October,2012. 12.HsiehCH,CheinLF2011Expressionofbamboomitochondrial-encodedNad1subunitinSaccharomycescerevisiae,The26thJointAnnualConferenceofBiomedicalSciences.Taipei,March,2011.#P1.. 13.ChienLF,LinCD,KouTT2010ProductionofH2bysolar-drivenpathwayaselectronsfromphotosyntheticelectrontransportchainbeingtransferringtohydrogenase,SatelliteMeetingofthe15thInternationalPhotosynthesisCongress,ISPR,Singapore,August,.pp.38. 14.LiuBH,ChienLF2010ConstructionoftheRNAinterferenceVectorstoknockdownPsbOproteininChlorellaspDT,The25thJointAnnualConferenceofBiomedicalSciences.Taipei,March,pp.434. 15.KouTT,ChienLF2010HomologousoverexpressionofhydrogenaseinChlorellasp.DTtoenhancehydrogenproduction,The25thJointAnnualConferenceofBiomedicalSciences.Taipei,March,2010,pp.449. 16.LinHD,ChienLF2009UsingRNAinterferencetogenerateD1-deficientorPsbO-deficientChlorellaspDTforhydrogenproduction,The24thJointAnnualConferenceofBiomedicalSciences.Taipei,March,,pp.735. 17.ChienLF,WuYC,KoGC2009MitochondrialproteomeinyoungbamboorhizomefromBambusaoldhamiiandPhyllostachysedulis,Abstract:PlantBiology.Hawaii,July,pp.45021. 18.ChiuCY,ChienLF2009ProteomicanalysisofmerAtransgenicChlorellabyIEF/SDS-PAGE,The24thJointAnnualConferenceofBiomedicalSciences.Taipei,March,pp.740. 19.WangSH,ChienLF2009Expressionandcharacterizationofbamboomitochondrial-encodedCox2subunitinyeast,The24thJointAnnualConferenceofBiomedicalSciences.Taipei,March,pp.736. 20.ChienLF,ChenHP,WuYC2008BamboomitochondrialenergymetabolicpathwaysinBambusaoldhamiiandPhyllostBiochimachysedulisduringrapidshootingstage,Acta-Bioenergetics1777:S98.#S13.41.15thEuropeanBioenergeticsConference,Dublin,Ireland,19-24thJuly2008. 21.TsaiHC,HsuYH,ChienLF2007Genomicanalysisofplantmitochondrialnd1andnd5subunitsofnadh:ubiquinoneoxidoreductase,The22ndJointAnnualConferenceofBiomedicalSciences.Taipei,March,pp.355. 22.HuangHS,ChangHY,ChenHP,LauYY,WuGW,ShiSH,TsaiHC,ChienLF2007Variationinphotosynthesis,respiration,andsuperoxidedismutaseactivityinBolboschoenusplanicu,The22ndJointAnnualConferenceofBiomedicalSciences.Taipei,March,pp.988. 23.ChienLF,ChenMW,HuangCC2006Mercuryphytoremediation:functionalexpressionofmercuricreductasefromBacillusmegateriumMB1iAbstract:PlantBiology2006,5-9August,Boston.pp.06024.